Effect of antiflagellar serum in the protection of mice against Clostridium chauvoei.

Production, characterization, and protective effect of monoclonal antibodies to Clostridium chauvoei flagella.

Monoclonal antibodies to flagella of Clostridium chauvoei were obtained by the fusion of murine myeloma cells (P3-X63-Ag8-U1) and spleen cells from BALB/c mice immunized with partially purified flagella of strain Okinawa. Enzyme-linked immunosorbent assay and Western blot analysis with partially purified flagella, flagellated cells, and nonflagellated mutants were used to show that five monoclo...

Sequencing and comparative analysis of flagellin genes fliA and fliB in bovine Clostridium chauvoei isolates

Background: Clostridium chauvoei is the etiological agent of blackleg as an endogenous infection in cattle.  Flagella have been known to play a critical role in the protective immunity of animals to clostridial infections. C.chauvoei has two copies of fliC gene, namely fliA and fliB. OBJECTIVES: The aim of this study was the determination and nucleotide sequence analysis of both copies of fliC ...

Large scale production of Blackleg vaccine by fermenter and enriched culture medium in Iran

In all biological systems growth is defined as increase of chemical compounds. Bacteria can achieve to balanced growth if they are growing in a medium, which are completely adapted to it. Clostridium chauvoei, (Clostridium feseri) is an anaerobic, spore forming, motile, and polymorph bacteria, which its size varies from 0.5-1 to 3-8 micron and could be observed as individual bacterium, diplo, a...

Preparation of concentrated blackleg vaccine

  Blackleg in cattle has been recognized since 1938 in Iran that mostly affects cattle in enzootic farm. Main object of this study was to prepare and formulate a concentrated potent vaccine for immunization of cattle against blackleg in Iran. Experimental concentrated blackleg vaccine was prepared according to the method described by FAO. The medium (Modified medium for production of experiment...

Characterization of Clostridium chauvoei strains isolated from an outbreak of blackleg in Iran